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Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. <t>ELISA</t> revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).
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Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. <t>ELISA</t> revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).
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Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. <t>ELISA</t> revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).
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Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. <t>ELISA</t> revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).
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Effect of fucoidan nanoparticles on IL-6 and <t> TNF- α </t> levels in the kidney tissue of streptozotocin-induced diabetic rats.
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Effect of fucoidan nanoparticles on IL-6 and <t> TNF- α </t> levels in the kidney tissue of streptozotocin-induced diabetic rats.
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Image Search Results


Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. ELISA revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).

Journal: Frontiers in Neuroscience

Article Title: Autism-Like Behavior in the Offspring of CYP11A1 -Overexpressing Pregnant Rats

doi: 10.3389/fnins.2021.774439

Figure Lengend Snippet: Primary microglial transcriptome and primary culture indicates immune activation in offspring from CYP11A1 -overexpressing dams. (A) Microglial cells from the offspring of AD-CYP11A1-treated pregnant rats were identified by their Cd11b positivity. (B) GO analysis revealed the activation of immune responses in primary microglia from the CYP11A1 overexpression group. (C) KEGG analysis indicated that GABAergic synapse related genes were enriched from the CYP11A1 overexpression group (over_represented p = 0.033). N = 3 for each group, and only male rats were used for the transcriptomic analysis. (D,E) Expression of the inflammatory tumor necrosis factor-alpha (TNF-α) was increased in the primary microglia in the CYP11A1 adenovirus infected cells. ELISA revealed TNF-α in the adenovirus infected culture medium (D) , and real-time PCR detected TNF-α mRNA in the microglia (E) (* P < 0.05).

Article Snippet: TNF-α levels were measured by rat TNF-α ELISA Kit (D731168, R&D Systems).

Techniques: Activation Assay, Over Expression, Expressing, Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Effect of fucoidan nanoparticles on IL-6 and  TNF- α  levels in the kidney tissue of streptozotocin-induced diabetic rats.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antioxidative Stress and Anti-Inflammatory Activity of Fucoidan Nanoparticles against Nephropathy of Streptozotocin-Induced Diabetes in Rats

doi: 10.1155/2022/3405871

Figure Lengend Snippet: Effect of fucoidan nanoparticles on IL-6 and TNF- α levels in the kidney tissue of streptozotocin-induced diabetic rats.

Article Snippet: Similarly, TNF- α was also measured with Rat TNF- α ELISA kit PicoKineTM Boster, according to the manufacturer protocol.

Techniques: Control